Photosynthetic and fluorescence analysis of chlorophyll deficient mutants of maize and tobacco

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Corn -- Analysis., Tobacco -- Anal
Statementby Eric Thomas Johnson.
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Three light intensity-dependent Chl b-deficient mutants, two in wheat and one in barley, were analyzed for their xanthophyll cycle carotenoids and Chl fluorescence characteristics under two different growth PFDs (30 versus μmol photonsm−2 s−1 incident light).

Mutants grown under low light possessed lower levels of total Chls and carotenoids per unit leaf area compared to wild type Cited by: Abstract.

Details Photosynthetic and fluorescence analysis of chlorophyll deficient mutants of maize and tobacco EPUB

Mutations that depress total chlorophyll content such as found in barley(l), maize(2), pea(3), soybean(4) and tobacco(5) generally result in an increased chlorophyll a: b ratio. Mutants of pea (3) and tobacco (5) have a decreased capacity for CO 2 assimilation, and the change in the chl a: b ratio in a mutant of maize (2) reflects a reduction in : Dimah Habash, Bernard Genty, Neil R.

Baker. Chlorophyll fluorescence is light re-emitted by chlorophyll molecules during return from excited to non-excited is used as an indicator of photosynthetic energy conversion in higher plants, algae and d chlorophyll dissipates the absorbed light energy by driving photosynthesis (photochemical energy conversion), as heat in non-photochemical quenching or by.

Miles D () Mutants of higher plants: maize. In A San Pietro, ed, Methods in Enzymology, Vol Academic Press, New York, pp 3–23; Miles D. The role of high chlorophyll fluorescence photosynthesis mutants in the analysis of chloroplast thylakoid membrane assembly and function.

Maydica.

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; –   ZHAO Hong-Bing et al. / Acta Agronomica Sinica,37(1): – Analysis of chlorophyll fluorescence kinetics is an in vivo technique that has been widely accepted to study photosynthetic capacity and changes in photosynthetic characteristics of chlorophyll-deficient mutants Cited by: 4.

Chlorophyll deficient mutants have been reported in and crops, like maize, pea, tobacco and barley [2 Chlorophyll a fluorescence analysis pointed to xan1 incompetence to fully express and.

One class of mutants in maize (Zea mays L.), hcf (high chlorophyll fluorescence), has been useful in providing clues about the organization, function, and biogenesis of specific membrane complexes within the thylakoid membranes of higher plants.

These seedling-lethal mutants are defective in electron flow through the photosynthetic complexes. All plants depend on the photosynthetic CO 2-fixing enzyme, Rubisco, to supply them with combinedalgae, and many bacteria have the so-called Form I Rubisco, which has a complex quaternary structure composed of eight large ( kD) subunits, which bear the active sites, and eight small ( kD) subunits (Roy and Andrews, ; Spreitzer and Salvucci, ).

Photosynthetic properties of maize hybrids under different environmental conditions probed by the chlorophyll a fluorescence Article (PDF Available) in. The annual Zea mays ssp. mexicana L. is a member of the teosinte group and a close wild relative of maize.

Thus, Zea mays ssp. mexicana L. can be effe. Principles of Modulated Fluorescence Analysis Chlorophyll fluorescence analysis allows noninvasive, near-instantaneous measurement of key aspects of photosynthetic light capture and electron transport.

Description Photosynthetic and fluorescence analysis of chlorophyll deficient mutants of maize and tobacco EPUB

For natural samples, flu-orescence signals are specific to photobionts and allow in situ measurements of small (61) or dilute (65, ) mixed. Measurements of chlorophyll fluorescence provide an elegant and non-invasive means of probing the dynamics of photosynthesis.

Advances in video imaging of chlorophyll fluorescence have now made it possible to study photosynthesis at all levels from individual cells to entire crop populations.

Since the technology delivers quantitative data, is easily scaled up and can be readily combined with. Chlorophyll fluorescence measurements (Mini-PAM) Chlorophyll a fluorescence measurements were performed with a Mini-PAM fluorometer (Heinz Walz GmBH, Effeltrich, Germany) equipped with a leaf clip holder either B (system for small size plants) or alternatively B (measurements on maize plants).

Two yellow rice mutants VG and VG were obtained during the tissue culture process from a rice plant (cv. Zhonghua No japonica) with inserted maize Ds transposon element. Absorption spectra and pigment composition showed that two mutants had no chlorophyll (Chl) b and lower Chl a content in comparison to the wild type (WT).

Net photosynthetic rate (P N), total electron transport rate. To evaluate the effects of light intensity on pigment content, HPLC analysis was carried out on extracts from normal and xan1 seedlings grown for 2 weeks under four different light intensities.

Total chlorophyll content of xan1 leaves grown under PPFD was similar to wild type ().A drastic reduction of chlorophyll levels was observed in xan1 leaves, with increasing light intensity. Chlorophyll a Fluorescence: A Signature of Photosynthesis highlights chlorophyll (Chl) a fluorescence as a convenient, non-invasive, highly sensitive, rapid and quantitative probe of oxygenic photosynthesis.

Thirty-one chapters, authored by 58 international experts, provide a solid foundation of the basic theory, as well as of the application of the rich information contained in the Chl a.

The photosynthetic, optical and morphological characteristics of a chlorophyll-deficient (Chl-deficient) “yellow” soybean mutant (MinnGold) were examined in comparison with two green varieties.

Chlorophyll a Fluorescence: A Signature of Photosynthesis highlights chlorophyll (Chl) a fluorescence as a convenient, non-invasive, highly sensitive, rapid and quantitative probe of oxygenic photosynthesis. Thirty-one chapters, authored by 58 international experts, provide a solid foundation of the basic theory, as well as of the application Reviews: 1.

Chlorophyll fluorescence analysis allows noninvasive, near-instantaneous measurement of key aspects of photosynthetic light capture and electron transport.

For natural samples, fluorescence signals are specific to photobionts and allow in situ measurements of small (61) or dilute (65, ) mixed natural populations. In addition, exogenous SNP application dramatically regulated chlorophyll α fluorescence kinetics e.g. the increase of maximum quantum yield of PSII (F v /F m), photosynthetic performance index (PI), and IP phase, whereas it remarkably reduced the polyphasic OJIP fluorescence transient, the accumulation of reactive oxygen species (H 2 O 2 and.

Chlorophyll fluorescence images were captured with a FluorCam MF imaging fluorometer (Photon Systems Instruments, Brno, Czech Republic) as described by Wingler et determination of F v /F m in dark-adapted plants, the leaves were illuminated with actinic light ( μmol m −2 s −1) and saturating flashes of s duration were applied every 2 min to determine NPQ.

Thirty-four recessive photosynthetic mutants of the high-chlorophyll-fluorescence (hcf) phenotype have been isolated by screening M2 progenies of ethyl methane sulfonate-treated seeds ofArabidopsis thaliana.

Most of the mutants isolated were found to be seedlinglethal, but could be grown on sucrose-supplemented media. Chlorophyll (Chl) fluorescence induction, absorption changes in. To begin the functional dissection of light signal transduction pathways of maize (Zea mays), we have identified and characterized the light-sensing mutant elm1 (elongated mesocotyl1).Seedlings homozygous for elm1 are pale green, show pronounced elongation of the mesocotyl, and fail to de-etiolate under red or far-red light.

Etiolated elm1 mutants contain no spectrally active phytochrome. Three different methods were used to measure photosynthesis: CO2 and H2O-vapour exchange, O2 evolution at a 5-kPa CO2 partial pressure, and chlorophyll fluorescence.

The three methods were. GENETICS OF PHOTOSYNTHESIS. Levine was amongst the first to use a genetic approach for studying the photosynthetic apparatus by isolating a large number of mutants deficient in photosynthetic activity (Levine, ).Although this approach was met with considerable skepticism at the beginning, it proved highly successful.

Tsuyoshi Endo's 63 research works with 4, citations and 3, reads, including: Overproduction of PGR5 enhances the electron sink downstream of photosystem I in a C 4 plant, Flaveria bidentis.

View More View Less. 1 Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Labora. Chlorophyll Fluorescence and Photosynthesis: The Basics. Annual Review of Plant Physiology and Plant Molecular Biology Figure 2: The principal features of photosynthetic electron transport under high light stress that lead to the production of ROS in chloroplasts and peroxisomes.

Two electron sinks can be used to alle. The role of chlorophyll b in photosynthesis: Hypothesis.-BMC Plant Biol. 1: 2, Falbel, T.G., Meehl, J.B., Staehelin, L.A.: Severity of mutant phenotype in a series of chlorophyll-deficient wheat mutants depends on light intensity and the severity of the block in chlorophyll synthesis.-Plant Physiol.

Go to original source. Chlorophyll fluorescence analysis is one of the most powerful and widely used techniques to study the effect of stresses on the photosynthetic process.

From the first utilization, the Fv/Fm ratio has been largely used as a sensitive indicator of plant photosynthetic performance. Decreases of this index are indicative of the reduction of photosystem II (PSII) efficiency, namely photoinhibition. such as water deficit, temperature, nutrient deficiency, polluting agents, attack by pathogens.

In this section, we present some examples from our own work to illustrate the use of chlorophyll fluorescence to investigate temperature stress in maize. The use of chlorophyll fluorescence to assess the allocation of electrons.Introduction.

Photosynthesis is the process of converting light energy to chemical energy and is the most important source of energy on the earth [].Chlorophyll (Chl) molecules harvest light energy for photosynthesis, so Chls are key cofactors for the photosynthetic apparatus [].The Chl biosynthesis pathway, including more than 17 enzymes in higher plants [3–7], comprises four distinct.Taken together, the chlorophyll fluorescence and pigment data for the pgp1 mutant most likely reflect the importance of PG in photosynthesis of seed plants.

This is consistent with previous observations on cyanobacterial mutants that are deficient in PG biosynthesis (Hagio et al., ; Sato et al., ). These cyanobacterial mutants have an.